Naturalists have long known that queens inside the hive emit two kinds of sound, called "tooting" and "quacking." A close analysis of these sounds and the circumstances of their emission now provides the strongest evidence that bees use sound to convey specific messages.
Tooting is the regal identification of a virgin queen soon after she has emerged from the cell in which she developed. A hive cannot tolerate more than one queen at a time. In a hive that lacks a queen several queen-bearing cells develop simultaneously in a comb, but one matures earlier than the others. Once this queen has emerged, has hardened and has become steady on her legs, she proceeds to visit other queen cells, tear them open and sting to death their potential but not yet mature queens. Often, however, the worker bees do not allow her to dispose of all her potential rivals in this way; they bar her from some of the cells. She then begins to toot and continues to do so day and night, perhaps for a week or more. Her tooting rises in intensity and sometimes can be heard more than 10 feet from the hive.
There's a video of piping queens after the jump.